Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease.

Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional growth factor that has profound regulatory effects on many developmental and physiological processes. Disruption of the TGF-beta 1 gene by homologous recombination in murine embryonic stem cells enables mice to be generated that carry the disrupted allele. Animals homozygous for the mutated TGF-beta 1 allele show no gross developmental abnormalities, but about 20 days after birth they succumb to a wasting syndrome accompanied by a multifocal, mixed inflammatory cell response and tissue necrosis, leading to organ failure and death. TGF-beta 1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions.     

The gene encoding ganglioside-induced differentiation-associated protein 1 is mutated in axonal Charcot-Marie-Tooth type 4A disease.

We identified three distinct mutations and six mutant alleles in GDAP1 in three families with axonal Charcot-Marie-Tooth (CMT) neuropathy and vocal cord paresis, which were previously linked to the CMT4A locus on chromosome 8q21.1. These results establish the molecular etiology of CMT4A (MIM 214400) and suggest that it may be associated with both axonal and demyelinating phenotypes.     

A New Local Search Algorithm for the Job Shop Scheduling Problem

0　IntroductionThejobshopschedulingproblemwithwhichwearecon cernedconsistsinschedulingasetofjobsonasetofma chinesfortheobjectiveofminimizingthemake span ,i.e .themaximumoftimeneededforfinishingalljobs,whichissubjecttotheconstrainsthateachjobhasafixedprocessingorderthroughthemachinesandeachmachinecanprocessatmostonejobatatime .ThisproblemisNP hardandevenisoneofthehardestcom binationaloptimizationproblems.Itiswellknownthatonlysmallsizeprobleminstancescanbesolvedwithinareasonablecomputa tionalti…     

模式语义链元建模及其应用

大粒度的模型转换是模型驱动软件开发场景下的重要技术需求，仅通过定义通用模型转换语言不足以满足这一需求，其中一个原因是通用模型转换语言工作在特定建模语言的元模型层次而非一般设计意图层次上．文中提出一种模式语义链(PSL)元建模方法．通过重型扩展统一建模语言(UML)元模型以直接形式化描述可表达一般设计意图的模式，并应用UML动作语义和元模型继承实现模式自动生成及转换．该方法为实现一船设计意图层次上的大粒度模型转换提供了有效支持．     

结合环的一个结构定理

本文讨论了一般结合环与(左)S-单式周期环在一定条件下的结构问题,得出它们分别是局部交换环与诣零交换环,或为局部交换环的亚直和。同时,推广和改进了文献[l]的结果。     

高阶延伸集和高阶延伸极限集的连通性

讨论动力系统中的高阶延伸集和高阶延伸极限集,给出集合的高阶延伸集和高阶延伸极限集的连通性的条件:设X是局部紧的度量空间,当M是连通的,D α(M)是紧的则它是连通的;当M是紧的和连通的,且J α(M)是紧的则它是连通的.     

基于概念图的超文本网页学习适应性机制研究

从概念图和超文本结构特点的相似性出发,分析了超文本网页的自适应方式和机制结构,提出适应性超文本链接的路径分类,对适应性超文本网页的结构设计提出了一些可行的建议.     

Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

散热器型芯烘烤窑净化系统中分离器的设计研究

为解决铸铁散热器生产环境污染、产品成品率及产品质量低下等问题,笔者研制了XWJ-1型消烟雾化净化系统。该系统是根据乡镇企业实际状况,在原烘烤窑内采用烟道重新配置、油烟预处理、水雾化冷却、分离污染物、净化排放五步骤设计。通过6年多的试验运行和改进,结果证明,使用XWJ-1型油烟雾化净化系统,可以把高温、高浓度烟气中的焦油和有害气体减少90%,并分离净化到符合国家环保排放标准要求(GB9078-1996,GB16297-1996),较好地解决了热能利用、污染物回收利用等问题,提高了制芯及散热器的成品率及其质量,使企业经济效益显著提高。     

篮球运动中运动员的机能恢复方法和措施

通过对篮球运动中运动员机能恢复方法手段进行研究,论述了科学、合理的方法、手段,对加快运动员体能恢复和提高运动能力的作用;提出了教育学、医学生物学、心理学等恢复方法的种类及应用的时机.